Aperçu rapide pour Chicken egg anti-Boeuf (Vache) IgG Anticorps (Agarose Beads) (ABIN1381626)
Antigène
IgG
Conjugué
Agarose Beads
Application
Separation (Sep)
Reactivité
Boeuf (Vache)
Hôte
Chicken egg
Attributs du produit
After multiple immunizations in Freunds adjuvant, eggs were collected and the IgY fraction prepared. The antibodies were immunoaffinity purified from a solid phase immunoabsorbant and conjugated to N-hydroxysuccinimide (NHS)-activated agarose beads. Beads were washed extensively after the blocking of the residual NHS sites.
The specific activity of the anti-BIG agarose was determined. For this lot, 1 mg bovine IgG bound per mL agarose.
Purification
egg yolk-derived purified IgY, affinity purified
Immunogène
Chickens were immunized with highly purified bovine IgG isolated from normal pooled bovine serum.
Indications d'application
Optimal working dilutions should be determined experimentally by the investigator.
Restrictions
For Research Use only
Format
Gel
Buffer
Phosphate buffered saline (PBS), pH 7.2
Agent conservateur
Sodium azide
Précaution d'utilisation
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Conseil sur la manipulation
Do not freeze. Sensitive to light. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.