Aperçu rapide pour Âne anti-Poulet IgY Anticorps (Agarose Beads) (ABIN1381671)
Antigène
IgY
Conjugué
Agarose Beads
Application
Separation (Sep)
Reactivité
Poulet
Hôte
Âne
Purification
Antibodies were isolated from immune sera using affinity chromatography and conjugated to N- hydroxysuccinimide (NHS)-activated agarose beads. Beads were washed extensively after the blocking of the residual NHS sites.
The specific activity of the anti-IgY-agarose was determined by applying 2.7 mg IgY in 24 mL PBS (0.1 mg/mL) to 1.0 mL bed volume of agarose. Two mL aliquots were collected. The total amount of IgY that bound to the column was calculated. For this lot, 1.3 mg IgY bound per mL agarose.
Immunogène
A donkey was immunized with highly purified chicken IgY.
Indications d'application
Optimal working dilutions should be determined experimentally by the investigator.
Restrictions
For Research Use only
Format
Gel
Buffer
Phosphate buffered saline (PBS), pH 7.2
Agent conservateur
Sodium azide
Précaution d'utilisation
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.