Âne anti-Mouton IgG (Heavy & Light Chain) Anticorps (PE) - Preadsorbed

N° du produit ABIN2669896

Détail du produit

Antigène: IgG

Fragment: F(ab')2 fragment

Épitope: Heavy & Light Chain

Reactivité: Mouton

Hôte: Âne

Clonalité: Polyclonal

Conjugué: PE

Application: Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Specificité: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Sheep IgG and Sheep Serum.

Réactivité croisée: Mouton

Attributs du produit: Anti-Sheep F(ab')2 IgG (H&L) phycoerythrin conjugated antibody generated in donkey detects specifically sheep IgG. This secondary phycoerythrin conjugated antibody anti-Sheep is ideal for investigators who routinely perform titration assays, microscopy and FACS analysis.
Anti-Sheep F(ab')2 IgG (H&L) phycoerythrin conjugated antibody generated in donkey detects specifically sheep IgG. This secondary phycoerythrin conjugated antibody anti-Sheep is ideal for investigators who routinely perform titration assays, microscopy and FACS analysis.

Purification: Preadsorption: Solid phase absorption

Immunogène:

Immunogen: Anti-Sheep IgG was produced by repeated immunization with sheep IgG whole molecule in donkey.

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Antibody Anti-Sheep F(ab')2 IgG (H&L) phycoerythrin conjugated is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody conjugate. Optimal titers for other applications should be determined by the researcher.

Flow Cytometry Dilution: 1:100 - 1:250

IF Microscopy Dilution: 1:100 - 1:250

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 0.5 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Product is photosensitive and should be protected from light.

Stock: RT,4 °C

Stockage commentaire: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody

Sujet:

Synonyms: Donkey F(ab')2 Anti-Sheep IgG Antibody Phycoerythrin Conjugation, Donkey F(ab')2 Anti-Sheep IgG PE Conjugated Antibody

Background: Anti-Sheep F(ab')2 IgG (H&L) phycoerythrin conjugated antibody generated in donkey detects specifically sheep IgG. This secondary phycoerythrin conjugated antibody anti-Sheep is ideal for investigators who routinely perform titration assays, microscopy and FACS analysis.