Aperçu rapide pour Chèvre anti-Souris Ig Anticorps (Biotin) - Preadsorbed (ABIN2689598)
Antigène
Ig
Clonalité
Polyclonal
Conjugué
Biotin
Application
ELISA, Flow Cytometry (FACS)
Isotype
Ig Fraction
Reactivité
Souris
Hôte
Chèvre
Attributs du produit
This polyclonal antibody reacts with whole molecule mouse IgG (heavy and light chains) and may also react with the light chains of other mouse immunoglobulins. This antibody has been reported to be reactive in ELISA with mouse IgG, IgM, and IgA. In addition, low level cross-reactivity with rat and hamseter Ig has been reported. Investigators should note that reactivity with some mouse IgM antibodies can be weak. Minimal cross-reaction to rat, human, bovine, horse and rabbit non-immunoglobulin serum proteins are expected. Note: This reagent may have considerable cross-reactivity on human peripheral blood leukocytes, contributing to undesired background signal, when used with the flow cytometric application. As a result, this product is not recommended for immunofluorescent staining and flow cytometric analysis of human cells. For this application, investigators may wish to consider FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988) as an alternative. For immunohistochemical staining, Biotin Goat Anti-Mouse Ig (Cat. No. 550337) is recommended.
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. The polyclonal antibody was purified from antiserum by negative adsorption and affinity chromatography.
Stock
4 °C
Stockage commentaire
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.