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Souris anti-Humain IgE Anticorps (FITC)

FACS Monoclonal 4H10 IgG1 FITC
N° du produit ABIN302010
  • Antigène Tous les produits IgE
    IgE
    Reactivité
    • 51
    • 31
    • 10
    • 10
    • 5
    • 4
    • 2
    • 1
    Humain
    Hôte
    • 58
    • 21
    • 11
    • 10
    • 4
    • 1
    Souris
    Clonalité
    • 66
    • 32
    Monoclonal
    Conjugué
    • 26
    • 17
    • 16
    • 15
    • 8
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    FITC
    Application
    • 55
    • 40
    • 30
    • 22
    • 20
    • 15
    • 12
    • 10
    • 10
    • 8
    • 8
    • 6
    • 5
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Specificité
    The mouse monoclonal antibody 4H10 reacts with human IgE, it recognizes an epitope different from the ones recognized by BE5 and 4G7 antibodies to IgE. The epitope is located within the amino acids 267-279 (TWLEDGQVMDVDL).
    Réactivité croisée (Details)
    Human
    Purification
    Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
    Immunogène
    Purified human IgE.
    Clone
    4H10
    Isotype
    IgG1
  • Indications d'application
    Flow cytometry: Recommended dilution: 4 μg/mL.
    Commentaires

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.1 mg/mL
    Buffer
    Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Do not freeze.
    Avoid prolonged exposure to light.
    Stock
    4 °C
    Stockage commentaire
    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Antigène
    IgE
    Abstract
    IgE Produits
    Sujet
    Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.,Immunoglobulin E
    Poids moléculaire
    180 kDa
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