Souris anti-Canard IgY Anticorps

N° du produit ABIN305009

Aperçu rapide pour Souris anti-Canard IgY Anticorps (ABIN305009)

Antigène: IgY

Clonalité: Monoclonal

Application: ELISA, Flow Cytometry (FACS), Western Blotting (WB)

Clone: 14A3

Isotype: IgG1

Détail du produit

Reactivité: Canard

Hôte: Souris

Specificité: Recognizes the light chain of duck immunoglobulin, shown to be expressed strongly on bursal cells and weakly on splenocytes and peripheral lymphoid cells, but not expressed by thymocytes. Double-staining with clone 14A3 and duck CD8 alpha clone Du CD8-1, revealed the presence of a CD8high /14A3- cytotoxic T cell population and a CD8low/14A3+ B cell population in duck spleen and also revealed a high percentage of CD8+ /14A3+ cells in duck bursa. The recognition of B cells by 14A3 was confirmed using a recently developed Flag-tagged chicken B cell marker, known as chBAFF-Flag (B cell activating factor). Recognizes IgA in Peking duck bile and can be used as a marker to identify B cells in both Peking and Muscovy duck and geese studies. Detects a band of approximately 25 kD in duck cell lysates in Western blotting.

Aucune reactivité croisée: Poulet

Attributs du produit: Antigene is shown to be expressed strongly on bursal cells and weakly on splenocytes and peripheral lymphoid cells, but not expressed by thymocytes. Double-staining with clone 14A3 and duck CD8 alpha clone Du CD8-1, revealed the presence of a CD8high /14A3- cytotoxic T cell population and a CD8low/14A3+ B cell population in duck spleen and also revealed a high percentage of CD8+ /14A3+ cells in duck bursa. The recognition of B cells by 14A3 was confirmed using a recently developed Flag-tagged chicken B cell marker, known as chBAFF-Flag (B cell activating factor). Recognizes IgA in Peking duck bile and can be used as a marker to identify B cells in both Peking and Muscovy duck and geese studies. Detects a band of approximately 25 kDa in duck cell lysates in Western blotting.

Purification: Affinity purified

Immunogène: Purified Peking duck yolk IgY.

Type of Immunogen: Purified protein

Information d'application

Indications d'application: Approved: ELISA, Flo, WB

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request.

Commentaires:

Target Species of Antibody: Duck

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.

Stock: -20 °C

Stockage commentaire: Short term: -20°C
Long term: -20°C.

Détail du antigène

Antigène: IgY

Classe de substances: Antibody

Poids moléculaire: 25 kDa