Souris anti-Humain IgE (Fc Region) Anticorps

N° du produit ABIN363694

Aperçu rapide pour Souris anti-Humain IgE (Fc Region) Anticorps (ABIN363694)

Antigène: IgE

Clonalité: Monoclonal

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)

Clone: Le27-NBS01

Isotype: IgG1 kappa

Détail du produit

Épitope: Fc Region

Reactivité: Humain

Hôte: Souris

Specificité: Human IgE, Fce4, no cross-ractivity with other human or murine Ab isotypes

Attributs du produit: IGHE, chromosome 14q32.33

Purification: Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B

Pureté: > 95 %, tested by analytical Gel Permeation Chromatography

Stérilité: Sterile filtered

Immunogène: purified human myeloma IgE

Information d'application

Indications d'application: For functional cellular assays (mast cell / basophil activation), in particular when used at dilutions less than 1:100 Quality assurance: Reactivity > 99 %, compared to calibrator (Internal Standard mAb Le27), tested by ELISA binding to immobilized human Standard IgE (SUS-11)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS, pH=7.2, sterile filtered, contains no NaN3

Agent conservateur: Azide free

Conseil sur la manipulation: Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.

Stock: 4 °C

Référence

Rudolf, Zuercher, Nechansky, Ruf, Vogel, Miescher, Stadler, Kricek: "Molecular basis for nonanaphylactogenicity of a monoclonal anti-IgE antibody." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 2, pp. 813-9, (2000) (PubMed).

Détail du antigène

Antigène: IgE

Classe de substances: Antibody

Sujet: The antibody recognizes an epitope within the Ce4 domain of human IgE. It does not block the binding of IgE to its high affinity receptor on mast cells and basophils and is therefore suitable to detect receptor bound IgE. It can also be used as cross-linking agent for allergen-independent triggering of mast cells and basophils.