Souris anti-Humain IgE (Fc Region) Anticorps

N° du produit ABIN363707

Aperçu rapide pour Souris anti-Humain IgE (Fc Region) Anticorps (ABIN363707)

Antigène: IgE

Clonalité: Monoclonal

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)

Clone: BSW17-NBS01

Isotype: IgG1 kappa

Détail du produit

Épitope: Fc Region

Reactivité: Humain

Hôte: Souris

Specificité: Human IgE, Fce3-4, no cross-ractivity with other human or murine Ab isotypes

Attributs du produit: IGHE, chromosome 14q32.33

Purification: Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B

Pureté: > 95 %, tested by analytical Gel Permeation Chromatography

Stérilité: Sterile filtered

Immunogène: purified human myeloma IgE

Information d'application

Indications d'application: For functional cellular assays (mast cell / basophil activation), when used at dilutions < 1:100, the sodium azide free formulation is recommended. Quality assurance: Reactivity > 99 %, compared to calibrator (Internal Standard mAb BSW17), tested by ELISA binding to immobilized human Standard IgE (SUS-11)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS, pH=7.2, sterile filtered, contains no NaN3

Agent conservateur: Azide free

Conseil sur la manipulation: Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.

Stock: 4 °C

Référence

Rudolf, Zuercher, Nechansky, Ruf, Vogel, Miescher, Stadler, Kricek: "Molecular basis for nonanaphylactogenicity of a monoclonal anti-IgE antibody." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 2, pp. 813-9, (2000) (PubMed).

Détail du antigène

Antigène: IgE

Sujet: The antibody recognizes a conformational epitope within the Ce3 domain of human IgE spanning towards the amino-terminal part of the Ce4 domain. It blocks the binding of IgE to its high affinity receptor on mast cells and basophils. It is therefore suited as an inhibitory reference compound for mast cell modulator profiling and basic research on IgE mediated mast cell receptor signaling.