ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Specificité
Reacts with the heavy chain of rabbit IgG as demonstrated by ELISA.
Attributs du produit
Source: Pooled antisera from goats hyperimmunized with normal rabbit IgG To insure lot-to-lot consistency, each batch of product is tested by ELISA, PCFIA and/or flow cytometry for conformance to characteristics of a standard reference reagent.
Purification
Affinity chromatography on rat IgG covalently linked to agarose
Working Dilution: 1:4,000-1:8,000 Representative data are included in this product insert.
Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions
For Research Use only
Format
Liquid
Conseil sur la manipulation
Avoid repeated freezing and thawing. Dilute only prior to immediate use Centrifuge product if not completely clear after standing at room temperature. Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Stock
4 °C
Beauchemin, Moerke, Faloon, Kaye: "Assay Development and High-Throughput Screening for Inhibitors of Kaposi's Sarcoma-Associated Herpesvirus N-Terminal Latency-Associated Nuclear Antigen Binding to Nucleosomes." dans: Journal of biomolecular screening, (2014) (PubMed).
Vistnes, Aronsen, Lunde, Sjaastad, Carlson, Christensen: "Pentosan Polysulfate Decreases Myocardial Expression of the Extracellular Matrix Enzyme ADAMTS4 and Improves Cardiac Function In Vivo in Rats Subjected to Pressure Overload by Aortic Banding." dans: PLoS ONE, Vol. 9, Issue 3, pp. e89621, (2014) (PubMed).
Mau, Kaiser, Suedekum: "Carbonic anhydrase II is secreted from bovine parotid glands." dans: Histology and histopathology, Vol. 25, Issue 3, pp. 321-9, (2010) (PubMed).
Basile, Friedrich, Spahic, Knipe, Mang, Leonard, Ashtiyani, Bacallao, Molitoris, Sutton: "Impaired endothelial proliferation and mesenchymal transition contribute to vascular rarefaction following acute kidney injury." dans: American journal of physiology. Renal physiology, (2010) (PubMed).