Souris anti-Humain IgE Anticorps (Biotin)

N° du produit ABIN457497

Aperçu rapide pour Souris anti-Humain IgE Anticorps (Biotin) (ABIN457497)

Antigène: IgE

Clonalité: Monoclonal

Conjugué: Biotin

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)

Clone: Le27-NBS01

Isotype: IgG1 kappa

Détail du produit

Reactivité: Humain

Hôte: Souris

Specificité: Human IgE, Fce4, no cross-ractivity with other human or murine Ab isotypes

Sensibilité: Reactivity > 95 %, compared to calibrator (Internal Standard mAb clone Le27), tested by ELISA binding to immobilized human Standard IgE (clone SUS-11)

Attributs du produit: IGHE, chromosome 14q32.33

Purification: Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B - rat monoclonal anti- mouse Ck

Pureté: > 95 %, tested by analytical Gel Permeation Chromatography

Immunogène: purified human myeloma IgE

Information d'application

Indications d'application: For functional cellular assays (mast cell / basophil activation), when used at dilutions < 1:100, the sodium azide free formulation (Cat. No. 0908-1-010-B) is recommended

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS, pH=7.2, 0.1 % NaN3

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Aliquot to Avoid repeated freezing and thawing.

Stock: 4 °C

Détail du antigène

Antigène: IgE

Sujet: The antibody recognizes an epitope within the Ce4 domain of human IgE. It does not block the binding of IgE to its high affinity receptor on mast cells and basophils and is therefore suitable to detect receptor bound IgE. It can also be used as cross-linking agent for allergen-independent triggering of mast cells and basophils.