Souris anti-Humain IgE Anticorps (Biotin)

N° du produit ABIN457499

Détail du produit

Antigène: IgE

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Biotin

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)

Specificité: Human IgE, Fce4, no cross-ractivity with other human or murine Ab isotypes

Sensibilité: Reactivity > 95 %, compared to calibrator (Internal Standard mAb clone Le27), tested by ELISA binding to immobilized human Standard IgE (clone SUS-11)

Attributs du produit: IGHE, chromosome 14q32.33

Purification: Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B - rat monoclonal anti- mouse Ck

Pureté: > 95 %, tested by analytical Gel Permeation Chromatography

Stérilité: Sterile filtered

Immunogène: purified human myeloma IgE

Clone: Le27-NBS01

Isotype: IgG1 kappa

Information d'application

Indications d'application: For functional cellular assays (mast cell / basophil activation), in particular when used at dilutions < 1:100

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS, pH=7.2, sterile filtered, contains no NaN3

Agent conservateur: Azide free

Conseil sur la manipulation: Aliquot to Avoid repeated freezing and thawing.

Stock: 4 °C

Détail du antigène

Antigène: IgE

Abstract: IgE Produits

Sujet: The antibody recognizes an epitope within the Ce4 domain of human IgE. It does not block the binding of IgE to its high affinity receptor on mast cells and basophils and is therefore suitable to detect receptor bound IgE. It can also be used as cross-linking agent for allergen-independent triggering of mast cells and basophils.