Souris anti-Humain IgE Anticorps (Biotin)

N° du produit ABIN457502

Aperçu rapide pour Souris anti-Humain IgE Anticorps (Biotin) (ABIN457502)

Antigène: IgE

Clonalité: Monoclonal

Conjugué: Biotin

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)

Clone: BSW17-NBS01

Isotype: IgG1 kappa

Détail du produit

Reactivité: Humain

Hôte: Souris

Specificité: Human IgE, Fce3-4, no cross-ractivity with other human or murine Ab isotypes

Sensibilité: Reactivity > 95 %, compared to calibrator (Internal Standard mAb clone BSW17), tested by ELISA binding to immobilized human Standard IgE (clone: SUS-11)

Attributs du produit: IGHE, chromosome 14q32.33

Purification: Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B - rat monoclonal anti- mouse Ck

Pureté: > 85 %, tested by analytical Gel Permeation Chromatography

Immunogène: purified human myeloma IgE

Information d'application

Indications d'application: When used for functional cellular assays at dilutions < 1:100, the Na-azide free formulation (Cat. No. 0910-1-010 / 050-B) is recommended

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS, pH=7.2, 0.1 % NaN3

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Aliquot to Avoid repeated freezing and thawing.

Stock: 4 °C

Détail du antigène

Antigène: IgE

Sujet: The antibody recognizes a conformational epitope within the Ce3 domain of human IgE spanning towards the amino-terminal part of the Ce4 domain. It blocks the binding of IgE to its high affinity receptor on mast cells and basophils. It is therefore suited as an inhibitory reference compound for mast cell modulator profiling and basic research on IgE mediated mast cell receptor signaling.