Chèvre anti-Chien IgM (Fc Region) Anticorps - Preadsorbed

N° du produit ABIN457680

Aperçu rapide pour Chèvre anti-Chien IgM (Fc Region) Anticorps - Preadsorbed (ABIN457680)

Antigène: IgM

Clonalité: Polyclonal

Application: ELISA, Western Blotting (WB), Dot Blot (DB), Indirect Immunofluorescence Assay (IFA)

Isotype: IgG

Détail du produit

Épitope: Fc Region

Reactivité: Chien

Hôte: Chèvre

Fonction: Goat anti Dog IgM (Fc specific)

Specificité: IgG fraction of polyclonal Goat antiSerum to Dog IgM, Fc specific

Réactivité croisée (Details): Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this immunoconjugate has not been tested in detail.

Attributs du produit: The reactivity of the antiserum is directed to the Fc subunit of the IgM molecule which expresses strict isotypic (class) specificity. It does not react with IgG, IgA and IgG/Fab or any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion. As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.

Purification: Purified IgG fraction from antiserum

Immunogène: Purified IgM isolated from dog serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Information d'application

Indications d'application: Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Détail du antigène

Antigène: IgM

Classe de substances: Antibody