Chèvre anti-Cobaye IgG1 (Subclass specific) Anticorps (HRP) - Preadsorbed

N° du produit ABIN457759

Aperçu rapide pour Chèvre anti-Cobaye IgG1 (Subclass specific) Anticorps (HRP) - Preadsorbed (ABIN457759)

Antigène: IgG1

Clonalité: Polyclonal

Conjugué: HRP

Application: ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Dot Blot (DB)

Isotype: IgG

Détail du produit

Épitope: Subclass specific

Reactivité: Cobaye

Hôte: Chèvre

Fonction: Goat anti Guinea Pig IgG (subclass specific), conjugated with Horseradish peroxidase

Specificité: Horseradish peroxidase-conjugated IgG fraction of polyclonal Goat antiSerum to Guinea Pig IgG1, subclass specific

Réactivité croisée (Details): This immunoconjugate is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this antiSerum has not been tested in detail.

Attributs du produit: The reactivity of the antiserum is directed to the subclass IgG1. It does not react with the other subclass of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in guinea pig serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG1 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to demonstrate circulating IgG1 antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of guinea pig origin known to be of the IgG1 isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgG1 in guinea pig serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Purification: Purified IgG fraction from antiserum

Immunogène: Pools of purified polyclonal IgG1 isolated from pooled guinea pig serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Information d'application

Indications d'application: ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: Peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Détail du antigène

Antigène: IgG1

Classe de substances: Antibody