Aperçu rapide pour Souris anti-Humain Bence-Jones Protein lambda (Chain lambda), (Free & Bound) Anticorps (ABIN457819)
Antigène
Bence-Jones Protein lambda
Clonalité
Monoclonal
Application
ELISA, Western Blotting (WB)
Clone
NI 412 and NI 268
Isotype
IgG1 kappa
Épitope
Chain lambda, Free & Bound
Reactivité
Humain
Hôte
Souris
Attributs du produit
The reactivity of the this preparation of two monoclonal antibodies is restricted to polyclonal and monoclonal immunoglobulins of the lambda type, as well as free lambda light chains as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immuno-peroxidase staining. Purified monoclonal mouse antibody to human Ig lambda chain, free and bound
Purification
Purified
Immunogène
Highly purified Bence Jones lambda proteins isolated from pooled human urine.
Indications d'application
To identify the light chain type of immunoglobulins or free light chains in human serum, other body fluids, cell and tissue substrates and to determine its concentration in indirect techniques as ELISA, indirect immunoperoxidasestaining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:200 upwards, in Western blotting from 1:400 upwards. These data should be interpreted as general recommendations only.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.
Concentration
0.4 mg/mL
Buffer
Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2).
Agent conservateur
Without preservative
Conseil sur la manipulation
Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4 °C, not refrozen, and preferably used the same day.
Stock
4 °C/-20 °C
Stockage commentaire
The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below - 24 °C.