Aperçu rapide pour Souris anti-Humain IgA6 Anticorps (ABIN457821)
Antigène
IgA6
Clonalité
Monoclonal
Application
Haemagglutination (H), Immunofluorescence (IF), ELISA, Western Blotting (WB)
Clone
NI 69-11 (A89-036)
Isotype
IgG1 kappa
Reactivité
Humain
Hôte
Souris
Specificité
The reactivity of the antiserum is restricted to a subclass specific determinant on the C H 2 domain of IgA1 as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). Purified monoclonal mouse antibody to human IgA1, subclass specific
Attributs du produit
Purified monoclonal mouse antibody to human IgA1, subclass specific
Purification
Purified
Immunogène
Highly purified monoclonal IgA1 isolated from human serum.
Indications d'application
To identify the presence of IgA1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immuno-peroxidase and immunofluorescence staining of cytoplasmic IgA1, haemagglutination and immunoblotting. The optimum working dilu-tion is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:200 upwards, in Western blotting from 1:500 upwards. These data should be interpreted as general recommendations only.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute the lyophilized ascites by adding 0. 5 mL sterile distilled water.
Concentration
0.4 mg/mL
Buffer
Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2)
Conseil sur la manipulation
Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
Stock
4 °C/-20 °C
Stockage commentaire
The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.