Souris anti-Humain IgG4 Anticorps

N° du produit ABIN457832

Aperçu rapide pour Souris anti-Humain IgG4 Anticorps (ABIN457832)

Antigène: IgG4

Clonalité: Monoclonal

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Clone: NI 315 (HP 6206)

Isotype: IgG1 kappa

Détail du produit

Reactivité: Humain

Hôte: Souris

Specificité: The reactivity of the antiserum is restricted to a subclass specific determinant on the Fc part of the IgG4 molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig. Monoclonal mouse antibody to human IgG4, subclass specific

Purification: Purified

Immunogène: Highly purified monoclonal IgG4 isolated from human serum

Information d'application

Indications d'application: To identify the presence of IgG4 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG4, and immunoblotting.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.

Concentration: 0.4 mg/mL

Buffer: Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2).

Agent conservateur: Without preservative

Conseil sur la manipulation: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Détail du antigène

Antigène: IgG4

Autre désignation: Immunoglobulin G4 (IgG4)

Classe de substances: Antibody