Souris anti-Humain IgA2 Anticorps (Biotin)

N° du produit ABIN457869

Aperçu rapide pour Souris anti-Humain IgA2 Anticorps (Biotin) (ABIN457869)

Antigène: IgA2

Clonalité: Monoclonal

Conjugué: Biotin

Application: Western Blotting (WB), ELISA

Clone: NI 512 (A89-038)

Isotype: IgG1 kappa

Détail du produit

Reactivité: Humain

Hôte: Souris

Specificité: The reactivity of the antiserum is restricted to a subclass specific determinant on IgA2 as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglu-tination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). Biotin-conjugated purified monoclonal mouse antibody to human IgA2, subclass specific

Attributs du produit: Biotin-conjugated purified monoclonal mouse antibody to human IgA2, subclass specific

Purification: Purified

Immunogène: Highly purified monoclonal IgA2 isolated from human serum.

Information d'application

Indications d'application: To identify the presence of IgA2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgA2, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used.
The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:250 upwards, in Western blotting from 1:500 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.

Concentration: 0.4 mg/mL

Buffer: Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline (pH 7.2).

Agent conservateur: Without preservative

Conseil sur la manipulation: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Détail du antigène

Antigène: IgA2