Souris anti-Humain IgA2 Anticorps (HRP)

N° du produit ABIN457871

Aperçu rapide pour Souris anti-Humain IgA2 Anticorps (HRP) (ABIN457871)

Antigène: IgA2

Clonalité: Monoclonal

Conjugué: HRP

Application: Western Blotting (WB), ELISA

Clone: NI 512 (A89-038)

Isotype: IgG1 kappa

Détail du produit

Reactivité: Humain

Hôte: Souris

Specificité: The reactivity of the antiserum is restricted to a subclass specific determinant on the IgA2 molecule as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). Horseradish peroxidase conjugated purified monoclonal mouse antibody to human IgA2, subclass specific

Attributs du produit: Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ=3.2).

Purification: Purified

Immunogène: Highly purified monoclonal IgA2 isolated from pooled human serum.

Information d'application

Indications d'application: To identify the presence of IgA2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immuno-peroxidase staining of cytoplasmic IgA2, and immunoblotting.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 to 1:100, in ELISA from 1:50 upwards, in Western blotting from 1:100 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.

Concentration: 0.4 mg/mL

Buffer: Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH 7.2).

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Détail du antigène

Antigène: IgA2