Souris anti-Humain IgG1 (Fc Region) Anticorps (Biotin)

N° du produit ABIN457885

Détail du produit

Antigène: IgG1

Épitope: Fc Region

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Biotin

Application: ELISA, Western Blotting (WB)

Specificité: The reactivity of the antiserum is restricted to the subclass specific determinant on the Fc portion of the IgG1 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immuno-precipitation and direct immunoperoxidase staining of cytoplasmic Ig.

Attributs du produit: Biotin-conjugated purified monoclonal mouse antibody to human IgG1, subclass specific
Biotin/IgG protein molar ratio (B/P) approximately 2.4.

Purification: Purified

Immunogène: Highly purified monoclonal IgG1 isolated from pooled human serum.

Clone: NI 132 (HP 6186)

Isotype: IgG1 kappa

Information d'application

Indications d'application: To identify the presence of IgG1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmicIgG1, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used.
The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:40, in ELISA from 1:100 to 1:2000, in Western blotting from 1:200 to 1:4000. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.

Concentration: 0.4 mg/mL

Buffer: Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline (pH 7.2).

Agent conservateur: Without preservative

Conseil sur la manipulation: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutionsshould be stored at +4 °C, not refrozen, and preferably used the same day.

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Détail du antigène

Antigène: IgG1

Abstract: IgG1 Produits

Classe de substances: Antibody