Souris anti-Humain IgM Anticorps (TRITC)

N° du produit ABIN457907

Aperçu rapide pour Souris anti-Humain IgM Anticorps (TRITC) (ABIN457907)

Antigène: IgM

Clonalité: Monoclonal

Conjugué: TRITC

Application: Immunofluorescence (IF)

Clone: NI 179

Isotype: IgG1 kappa

Détail du produit

Reactivité: Humain

Hôte: Souris

Specificité: The reactivity of the antiserum is restricted to a isotype specific determinant of the IgM molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immuno-precipitation and direct immunofluorescence staining of cytoplasmic Ig. Tetramethylrhodamine isothiocyanate-conjugated purified monoclonal mouse antibody to human IgM, isotype specific

Attributs du produit: Tetramethylrhodamine isothiocyanate-conjugated purified monoclonal mouse antibody to human IgM, isotype specific
Fluorochrome/IgG protein molar ratio (E/P) approximately 5.7.

Purification: Purified

Immunogène: Highly purified IgM isolated from human serum.

Information d'application

Indications d'application: To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect immunofluorescence staining of cytoplasmic IgM and cell membrane IgM.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100. These data should be interpreted as general recommendations only.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.

Concentration: 0.4 mg/mL

Buffer: Purified monoclonal mouse IgG1 kappa conjugated with TRITC, lyophilized from a solution in phosphate buffered saline (pH 7.2).

Agent conservateur: Without preservative

Conseil sur la manipulation: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Détail du antigène

Antigène: IgM

Classe de substances: Antibody