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Souris anti-Humain IgA2 (Allotype IgA2(m)2) Anticorps

ELISA, IF Monoclonal NI 194-3 (A89-040) IgG1 unconjugated
N° du produit ABIN457924

Aperçu rapide pour Souris anti-Humain IgA2 (Allotype IgA2(m)2) Anticorps (ABIN457924)

Antigène

IgA2

Clonalité

  • 11
  • 2
Monoclonal

Application

  • 10
  • 5
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
ELISA, Immunofluorescence (IF)

Clone

NI 194-3 (A89-040)

Isotype

IgG1
  • Épitope

    • 4
    • 3
    • 1
    • 1
    Allotype IgA2(m)2

    Reactivité

    Humain

    Hôte

    • 11
    • 2
    Souris

    Specificité

    Monoclonal Mouse antiSerum to Human IgA2(m)2, allotype specific

    Réactivité croisée (Details)

    The antiSerum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species.

    Attributs du produit

    The reactivity of the antiserum is restricted to the IgA2(m)2 Molecule as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). To identify the presence of IgA2(m)2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining of cytoplasmic IgA2(m)2, haemagglutination and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:200 upwards, in Western blotting from 1:500 upwards. These data should be interpreted as general recommendations only.

    Purification

    Delipidated, heat inactivated, stable ascites

    Stérilité

    Heat-inactivated

    Immunogène

    Highly purified monoclonal IgA2(m)2 isolated from human serum.
  • Indications d'application

    Indirect immunofluorescence,ELISA.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Reconstitute the lyophilized ascites by adding 0.5 mL sterile distilled water.Delipidated, heat inactivated, lyophilized, stable whole ascites IgG concentration is 1 mg/mL. No foreign proteins added. Reconstitute the lyophilized ascites by adding 0.5 mL sterile distilled water.

    Buffer

    Delipidated, heat inactivated, lyophilized, stable whole ascites IgG concentration is 1 mg/mL. No foreign proteins added.

    Stock

    RT,4 °C,-20 °C

    Stockage commentaire

    The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4°C, not refrozen, and preferably used the same day.
  • Antigène

    IgA2

    Autre désignation

    IgA22
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