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Chèvre anti-Humain IgG (Heavy & Light Chain) Anticorps

ICC, ID, IEP, RID Polyclonal IgG unconjugated
N° du produit ABIN457952

Aperçu rapide pour Chèvre anti-Humain IgG (Heavy & Light Chain) Anticorps (ABIN457952)

Antigène

IgG

Clonalité

  • 3132
  • 148
  • 6
  • 1
Polyclonal

Application

  • 1724
  • 1701
  • 1683
  • 1631
  • 920
  • 873
  • 802
  • 403
  • 351
  • 232
  • 232
  • 106
  • 87
  • 65
  • 54
  • 33
  • 24
  • 19
  • 18
  • 18
  • 16
  • 16
  • 12
  • 9
  • 8
  • 8
  • 7
  • 4
  • 4
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Immunocytochemistry (ICC), Immunodiffusion (ID), Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)

Isotype

IgG
  • Épitope

    • 2332
    • 314
    • 286
    • 136
    • 133
    • 67
    • 53
    • 29
    • 28
    • 28
    • 19
    • 5
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Heavy & Light Chain

    Reactivité

    • 638
    • 566
    • 551
    • 404
    • 360
    • 232
    • 190
    • 155
    • 134
    • 125
    • 111
    • 88
    • 86
    • 85
    • 83
    • 62
    • 48
    • 31
    • 28
    • 18
    • 16
    • 10
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain

    Hôte

    • 1553
    • 1382
    • 551
    • 207
    • 98
    • 84
    • 38
    • 29
    • 21
    • 20
    • 16
    • 11
    • 11
    Chèvre

    Specificité

    The reactivity of the antiserum is directed to the Fc and Fab subunits of IgG. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. In immunoelectrophoresis against human serum a single characteristic precipitin line with a spur is usually obtained.

    Attributs du produit

    Total protein and IgG concentration in the antiserum are comparable to those of pooled goat serum. Antibody titre: Precipitin titre not less than 1:64 when tested against normal human serum in agar block titration.

    Purification

    Delipidated, heat inactivated lyophilized stable whole serum
    No adsorption required.

    Immunogène

    Purified normal IgG isolated from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
  • Indications d'application

    In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG, as secondary antibody to precipitate the immunoglobulin in normal human serum, to prepare an immunoadsorbent for the production of Ig-free human serum, plasma or non-Ig preparations. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay reagents meeting the necessary additional specificity and performance requirements are available.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    It is reconstituted by adding 1 mL sterile distilled water.

    Buffer

    No foreign proteins added.

    Agent conservateur

    Without preservative

    Conseil sur la manipulation

    Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same da y. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
  • Antigène

    IgG

    Classe de substances

    Antibody
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