Chèvre anti-Humain IgG (Heavy & Light Chain) Anticorps

N° du produit ABIN457953

Aperçu rapide pour Chèvre anti-Humain IgG (Heavy & Light Chain) Anticorps (ABIN457953)

Antigène: IgG

Clonalité: Polyclonal

Application: Immunoprecipitation (IP)

Détail du produit

Épitope: Heavy & Light Chain

Reactivité: Humain

Hôte: Chèvre

Specificité: Precipitating polyclonal Goat antiSerum to Human IgG, heavy and light chains

Réactivité croisée (Details): Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. This antiSerum has been tested for cross-reactivity in double radial immunodiffusion with the following results: Bovine - Dog + Horse ± pigeon - Sheep - Cat + Guinea Pig + Monkey ++ Rabbit + Swine + Chicken - Hamster ± Mouse ± Rat + Turkey -

Attributs du produit: The reactivity of the antiserum is directed to the Fc and Fab subunits of IgG. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. In immunoelectrophoresis against human serum a single characteristic precipitin line with a spur is usually obtained. It does not react with any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG, as secondary antibody to precipitate the immunoglobulin in normal human serum, to prepare an immunoadsorbent for the production of Ig-free human serum, plasma or non-Ig preparations. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay reagents meeting the necessary additional specificity and performance requirements are available.

Purification: Delipidated, heat inactivated, stable whole serum

Stérilité: Heat-inactivated

Immunogène: Purified normal IgG isolated from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Information d'application

Indications d'application: In immunoelectrophoresis use 2 μL or equivalent against 120 μL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μL antiserum in a 3 mm diameter centre well and 2 μL serum samples (neat and diluted) in 2 mm diameter peripheral wells.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water.Delipidated, heat inactivated lyophilized stable whole serum No preservative added, as it may interfere with the antibody activity. No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled goat serum. No foreign proteins added. It is reconstituted by adding 1 mL sterile distilled water.

Buffer: Delipidated, heat inactivated lyophilized stable whole serum No preservative added, as it may interfere with the antibody activity. No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled goat serum.

Agent conservateur: Without preservative

Stock: RT,4 °C,-20 °C

Stockage commentaire: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Détail du antigène

Antigène: IgG

Classe de substances: Antibody