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Chèvre anti-Humain IgA, IgG, IgM (Fc Region) Anticorps

ICC, ID, IEP, RID Polyclonal IgG unconjugated
N° du produit ABIN457978

Aperçu rapide pour Chèvre anti-Humain IgA, IgG, IgM (Fc Region) Anticorps (ABIN457978)

Antigène

IgA, IgG, IgM

Clonalité

  • 187
  • 2
Polyclonal

Application

  • 104
  • 102
  • 73
  • 67
  • 66
  • 36
  • 23
  • 22
  • 20
  • 17
  • 16
  • 13
  • 12
  • 11
  • 10
  • 10
  • 2
  • 1
  • 1
  • 1
Immunocytochemistry (ICC), Immunodiffusion (ID), Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)

Isotype

IgG
  • Épitope

    • 51
    • 31
    • 16
    • 8
    • 5
    Fc Region

    Reactivité

    • 63
    • 39
    • 27
    • 25
    • 12
    • 7
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Humain

    Hôte

    • 131
    • 55
    • 4
    • 3
    • 2
    Chèvre

    Specificité

    The reactivity of the antiserum is directed to the Fc subunits of the major immuno-globulins.

    Attributs du produit

    Total protein and IgG concentration in the antiserum are comparable to those of pooled goat serum. Antibody titre: Precipitin titre not less than 1:64 when tested against normal human serum in agar block titration.

    Purification

    Delipidated, heat inactivated, lyophilized stable whole serum
    Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

    Immunogène

    Purified normal IgG, IgA and IgM fractions isolated from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
  • Indications d'application

    In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins, as secondary antibody to precipitate the immunoglobulin in normal human serum. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    It is reconstituted by adding 1 mL sterile distilled water.

    Buffer

    No foreign proteins added.

    Agent conservateur

    Without preservative

    Conseil sur la manipulation

    Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
  • Antigène

    IgA, IgG, IgM

    Autre désignation

    IgG + IgA + IgM

    Classe de substances

    Antibody
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