Chèvre anti-Humain IgA, IgG, IgM (Fab Region) Anticorps (TRITC) - Preadsorbed

N° du produit ABIN458002

Aperçu rapide pour Chèvre anti-Humain IgA, IgG, IgM (Fab Region) Anticorps (TRITC) - Preadsorbed (ABIN458002)

Antigène: IgA, IgG, IgM

Clonalité: Polyclonal

Conjugué: TRITC

Application: Immunofluorescence (IF)

Isotype: IgG

Détail du produit

Épitope: Fab Region

Reactivité: Humain

Hôte: Chèvre

Specificité: The reactivity of the antiserum is directed to the major isotypes of the human immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins

Attributs du produit: Tetramethylrhodamine isothiocyanate-conjugated IgG fraction of polyclonal goat antiserum to human IgG, IgA and IgM, heavy and light chains
Fluorochrome/IgG protein molar ratio (F/P) is approximately 1.5.

Purification: Preadsorption: Immunoaffinity adsorbed using insolubilized antigens.

Immunogène: Purified immunoglobulin fractions containing IgG, IgA and IgM isolated from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Information d'application

Indications d'application: Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates, to demonstrate immunoglobulins or specific antibodies in cells and tissues, to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water. Spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.

Concentration: 10 mg/mL

Buffer: TRITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Agent conservateur: Without preservative

Conseil sur la manipulation: Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, a nd preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Détail du antigène

Antigène: IgA, IgG, IgM

Autre désignation: IgG + IgA + IgM

Classe de substances: Antibody