Lapin anti-Singe IgA, IgG, IgM Anticorps - Preadsorbed

N° du produit ABIN458325

Aperçu rapide pour Lapin anti-Singe IgA, IgG, IgM Anticorps - Preadsorbed (ABIN458325)

Antigène: IgA, IgG, IgM

Clonalité: Polyclonal

Application: ELISA, Western Blotting (WB), Immunocytochemistry (ICC)

Isotype: IgG

Détail du produit

Reactivité: Singe

Hôte: Lapin

Specificité: The defined antibody specificity is directed to the major isotypes of the monkey immunoglobulin system and to the surface determinants of the common Fab portion as tested against as tested in immunoelectrophoresis and double radial immunodiffusion (Ouchterlony).

Attributs du produit: Purified IgG fraction of polyclonal rabbit antiserum to monkey immunoglobulins IgG, IgA, IgM, heavy and light chains

Purification: Preadsorption: Immunoaffinity adsorbed using insolubilized Ig-depleted monkey serum.

Immunogène: Highly purified IgG, IgA and IgM isolated from Rhesus monkey serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Information d'application

Indications d'application: The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates, in radioimmunoassay, for the production of immunoconjugates with a selected marker, to prepare immunoaffinity adsorbents by coupling to an artificial carrier, in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent, in Western blotting. This product is not pre-diluted.
The optimum working dilution of each product should be established by titration before being used. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250, in ELISA and comparable non-precipitating antibody-binding assays are between 1:1,000 and 1:5,000.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.

Concentration: 10 mg/mL

Buffer: Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Agent conservateur: Without preservative

Conseil sur la manipulation: Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Détail du antigène

Antigène: IgA, IgG, IgM

Autre désignation: IgG + IgA + IgM

Classe de substances: Antibody