Chèvre anti-Souris IgG1, IgG2a, IgG2b, IgG3 (Fc Region) Anticorps

N° du produit ABIN458342

Aperçu rapide pour Chèvre anti-Souris IgG1, IgG2a, IgG2b, IgG3 (Fc Region) Anticorps (ABIN458342)

Antigène: IgG1, IgG2a, IgG2b, IgG3

Clonalité: Polyclonal

Application: Immunoprecipitation (IP)

Détail du produit

Épitope: Fc Region

Reactivité: Souris

Hôte: Chèvre

Specificité: Precipitating polyclonal Goat antiSerum to Mouse IgG, Fc specific

Réactivité croisée (Details): Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has been tested in double radial immunodiffusion anti several sera with the following results: cow - Duck - Horse - Rat + Cat - Guinea Pig+ Monkey - Sheep - Chicken - Human - pigeon - Swine - Dog - Hamster ± Rabbit - Turkey -

Attributs du produit: The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule. The antiserum contains antibodies to subclass-specific determinants as well as to determinants shared by two or more subclasses of IgG. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against normal mouse plasma, serum, and immunoglobulin fractions, no reaction is obtained with IgA, IgM and IgG/Fab fragments or any other serum protein. In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG, as secondary antibody to precipitate the immunoglobulin in normal mouse serum, to prepare an immunoadsorbent for the production of Ig-free mouse serum, plasma or non-Ig preparations.

Purification: Delipidated, heat inactivated, stable whole serum

Stérilité: Heat-inactivated

Immunogène: Purified normal IgG, including all known subclasses, isolated from pooled mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Information d'application

Indications d'application: In immunoelectrophoresis use 2 μL or equivalent against 120 μL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μL antiserum in a 3 mm diameter centre well and 2 μL serum samples (neat and diluted) in 2 mm diameter peripheral wells.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water.Delipidated, heat inactivated, lyophilized stable whole serum. No preservative added, as it may interfere with the antibody activity. Total protein and IgG concentration in the antiserum are comparable to those of pooled goat serum. No foreign proteins added. It is reconstituted by adding 1 mL sterile distilled water.

Buffer: Delipidated, heat inactivated, lyophilized stable whole serum. No preservative added, as it may interfere with the antibody activity. Total protein and IgG concentration in the antiserum are comparable to those of pooled goat serum. No foreign proteins added.

Agent conservateur: Without preservative

Stock: RT,4 °C,-20 °C

Stockage commentaire: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Détail du antigène

Antigène: IgG1, IgG2a, IgG2b, IgG3

Autre désignation: IgG1 IgG2a IgG2b IgG3