Chèvre anti-Souris IgG2 (Subclass specific) Anticorps (Biotin) - Preadsorbed

N° du produit ABIN458402

Aperçu rapide pour Chèvre anti-Souris IgG2 (Subclass specific) Anticorps (Biotin) - Preadsorbed (ABIN458402)

Antigène: IgG2

Clonalité: Polyclonal

Conjugué: Biotin

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Dot Blot (DB)

Isotype: IgG

Détail du produit

Épitope: Subclass specific

Reactivité: Souris

Hôte: Chèvre

Fonction: Goat anti Mouse IgG2 (subclass specific), conjugated with Biotin

Specificité: Biotin-conjugated IgG fraction of polyclonal Goat antiSerum to Mouse IgG2, subclass specific

Réactivité croisée (Details): This immunoconjugate is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. However this conjugate has been passed over appropriate immunoadsorbents to remove antibodies cross-reacting with Human immunoglobulins. This renders it specific for use in test systems containing material of Human origin (e.g. Human tissue/Mouse monoclonal antibody to a Human tissue constituent/anti Mouse Ig isotype-specific immunoconjugate).

Attributs du produit: The reactivity of the antiserum is directed to the subclasses IgG2a and IgG2b. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion In immunocytochemical and immunohistochemical staining for the detection of IgG2 at the cellular and subcellular level of appropriately treated cell and tissue substrates, to demonstrate circulating IgG2a antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of mouse origin known to be of the IgG2 isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgG2 in mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user's choice has to be used This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Purification: Purified

Immunogène: Pools of purified homogenous IgG2a and IgG2b isolated from mouse serum of strains belonging to the allelic types Igh-1a an Igh-1b. Freund's complete adjuvant is used in the first step of the immunization procedure.

Information d'application

Indications d'application: ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: Biotin-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Détail du antigène

Antigène: IgG2

Classe de substances: Antibody