Lapin anti-Souris IgE (Fc Region) Anticorps

N° du produit ABIN458429

Détail du produit

Antigène: IgE

Épitope: Fc Region

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Application: Immunoprecipitation (IP)

Specificité: Precipitating polyclonal Rabbit antiSerum to Mouse IgE, Fc specific

Réactivité croisée (Details): This IgG fraction is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this product has not been tested in detail because of the low level of IgE in most species..

Attributs du produit: The reactivity of the antiserum is directed to the Fc subunits of IgE as tested against mouse sera and a panel of purified homogenous immunoglobulins. It does not react with IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of homogenous IgE in mouse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of mouse IgE from serum or plasma.

Purification: Delipidated, heat inactivated, stable whole serum

Stérilité: Heat-inactivated

Immunogène: Pools of purified homogenous IgE isolated from pooled mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Information d'application

Indications d'application: In immunoelectrophoresis use 2 μL or equivalent against 120 μL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μL antiserum in a 3 mm diameter centre well and 2 μL serum samples (neat and diluted) in 2 mm diameter peripheral wells.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.Delipidated, heat inactivated, lyophilized stable whole serum No preservative added. Total protein and IgG concentration in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.

Buffer: Delipidated, heat inactivated, lyophilized stable whole serum No preservative added. Total protein and IgG concentration in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added.

Agent conservateur: Without preservative

Stock: RT,4 °C,-20 °C

Stockage commentaire: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Détail du antigène

Antigène: IgE

Abstract: IgE Produits