Lapin anti-Souris IgG2 Anticorps (TRITC) - Preadsorbed

N° du produit ABIN458468

Aperçu rapide pour Lapin anti-Souris IgG2 Anticorps (TRITC) - Preadsorbed (ABIN458468)

Antigène: IgG2

Clonalité: Polyclonal

Conjugué: TRITC

Application: Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Isotype: IgG

Détail du produit

Reactivité: Souris

Hôte: Lapin

Specificité: Specificity of this product has not been tested in detail.

Purification: Preadsorption: Immunoaffinity adsorbed using insolubilized antigens.

Immunogène: Pools of purified homogenous IgG2a and IgG2b isolated from mouse serum of strains belonging to the allelic types Igh-1a and Igh-1b. Freund's complete adjuvant is used in the first step of the immunization procedure.

Information d'application

Indications d'application: In immunocytochemical and immunohistochemical single and double staining for the detection of IgG2 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, as indicating second antibody conjugate to analyze or separate human cells after reacting with monoclonal mouse antibodies in immunofluorescence microscopy, fluorescence activated cell sorting and flow cytometry, to identify and measure IgG2 in mouse serum or other body fluids. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40, depending on the method used.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.

Concentration: 10 mg/mL

Buffer: TRITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Agent conservateur: Without preservative

Conseil sur la manipulation: Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Détail du antigène

Antigène: IgG2

Classe de substances: Antibody