Lapin anti-Souris IgG2b Anticorps (TRITC) - Preadsorbed

N° du produit ABIN458470

Aperçu rapide pour Lapin anti-Souris IgG2b Anticorps (TRITC) - Preadsorbed (ABIN458470)

Antigène: IgG2b

Clonalité: Polyclonal

Conjugué: TRITC

Application: Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Isotype: IgG

Détail du produit

Reactivité: Souris

Hôte: Lapin

Specificité: Specificity of this product has not been tested in detail.

Purification: Preadsorption: Immunoaffinity adsorbed using insolubilized antigens.

Immunogène: Pools of purified homogenous IgG2b isolated from pooled mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Information d'application

Indications d'application: In immunocytochemical and immunohistochemical staining for the detection of IgG2b at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to identify and measure IgG2b in mouse serum or other body fluids. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:30, depending on the method used.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.

Concentration: 10 mg/mL

Buffer: TRITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Agent conservateur: Without preservative

Conseil sur la manipulation: Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Détail du antigène

Antigène: IgG2b

Classe de substances: Antibody