Chèvre anti-Lapin IgG (Fc Region) Anticorps

N° du produit ABIN458591

Aperçu rapide pour Chèvre anti-Lapin IgG (Fc Region) Anticorps (ABIN458591)

Antigène: IgG

Clonalité: Polyclonal

Application: Immunoprecipitation (IP)

Détail du produit

Épitope: Fc Region

Reactivité: Lapin

Hôte: Chèvre

Specificité: Precipitating polyclonal Goat antiSerum to Rabbit IgG, Fc specific

Réactivité croisée (Details): The antiSerum does not cross-react with any other component of the Rabbit immunoglobulin system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

Attributs du produit: The reactivity of the antiserum is restricted to the Fc part of the IgG molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal rabbit serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG. No precipitation reaction is obtained with purified IgA, IgM, and IgG/Fab fragments. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG in rabbit serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of rabbit IgG from serum or plasma.

Purification: Delipidated, heat inactivated, stable whole serum

Stérilité: Heat-inactivated

Immunogène: Highly purified normal IgG isolated from pooled rabbit serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Information d'application

Indications d'application: In immunoelectrophoresis use 2 μL serum or equivalent against 120 μL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μL antiserum in 3 mm diameter center well and 2 μL serum samples (neat and serially diluted in 2 mm diameter peripheral wells.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.Delipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added. It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.

Agent conservateur: Without preservative

Stock: RT,4 °C,-20 °C

Stockage commentaire: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Détail du antigène

Antigène: IgG

Classe de substances: Antibody