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Chèvre anti-Rat IgG2b (Fc Region) Anticorps

ID, IEP Polyclonal IgG unconjugated
N° du produit ABIN458649

Aperçu rapide pour Chèvre anti-Rat IgG2b (Fc Region) Anticorps (ABIN458649)

Antigène

IgG2b

Clonalité

  • 74
  • 21
Polyclonal

Application

  • 59
  • 48
  • 37
  • 34
  • 32
  • 21
  • 14
  • 9
  • 8
  • 7
  • 5
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
Immunodiffusion (ID), Immunoelectrophoresis (IEP)

Isotype

IgG
  • Épitope

    • 33
    • 8
    • 4
    • 3
    • 2
    • 1
    Fc Region

    Reactivité

    • 93
    • 23
    Rat

    Hôte

    • 61
    • 33
    • 13
    • 8
    • 1
    Chèvre

    Specificité

    The reactivity of the antiserum is directed to the Fc portion of the subclass IgG2b.

    Attributs du produit

    Total protein and IgG concentration in the antiserum are comparable to those of pooled normal goat serum. Antibody titre: Precipitin titre not less then 1:32 when tested against pooled normal rat serum in agar block immunodiffus

    Purification

    Delipidated, heat inactivated, lyophilized whole antiserum.
    Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

    Immunogène

    Pools of purified homogenous IgG2b isolated from pooled rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
  • Indications d'application

    In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG2b in rat serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of rat IgG2b from serum or plasma.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.

    Buffer

    No foreign proteins added.

    Agent conservateur

    Without preservative

    Conseil sur la manipulation

    Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4 °C, not refrozen, and preferably used the same day.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
  • Antigène

    IgG2b

    Classe de substances

    Antibody
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