Chèvre anti-Rat IgA, IgG, IgM Anticorps (Biotin) - Preadsorbed

N° du produit ABIN458680

Aperçu rapide pour Chèvre anti-Rat IgA, IgG, IgM Anticorps (Biotin) - Preadsorbed (ABIN458680)

Antigène: IgA, IgG, IgM

Clonalité: Polyclonal

Conjugué: Biotin

Application: ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Isotype: IgG

Détail du produit

Reactivité: Rat

Hôte: Chèvre

Specificité: The reactivity of the antiserum is directed to the major isotypes of the rat immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal rat plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained.

Attributs du produit: Biotin-conjugated IgG fraction of polyclonal goat antiserum to rat IgG, IgA and IgM, heavy and light chains, adsorbed for human and mouse cross-reactivity.
Biotin/IgG protein molar ratio (B/P) is approximately 6.7.

Purification: Preadsorption: Immunoaffinity adsorbed using insolubilized Ig-depleted rat serum as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. This product has been passed over appropriate immunoadsorbent to remove antibodies cross-reacting with human and mouse immunoglobulins. This renders it specific for use in test systems containing material of human origin. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.

Immunogène: Purified polyclonal rat IgG, and homogenous IgA and IgM isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Information d'application

Indications d'application: In immunocytochemical and immunohistochemical staining of monoclonal rat monoclonal antibodies in the presence of human cell or serum components at the cellular and subcellular level. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:50 and 1:250, in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:8,000.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water. Spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.

Concentration: 10 mg/mL

Buffer: Biotin-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Agent conservateur: Without preservative

Conseil sur la manipulation: Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Détail du antigène

Antigène: IgA, IgG, IgM

Autre désignation: IgG + IgA + IgM

Classe de substances: Antibody