Mouton anti-Rat IgG1 (Subclass specific) Anticorps

N° du produit ABIN458733

Aperçu rapide pour Mouton anti-Rat IgG1 (Subclass specific) Anticorps (ABIN458733)

Antigène: IgG1

Clonalité: Polyclonal

Application: Immunoelectrophoresis (IEP), Ouchterlony Double Diffusion (ODD)

Isotype: IgG

Détail du produit

Épitope: Subclass specific

Reactivité: Rat

Hôte: Mouton

Fonction: Sheep anti Rat IgG1 (subclass specific)

Specificité: Precipitating polyclonal Sheep antiSerum to Rat IgG1, subclass specific.

Réactivité croisée (Details): This IgG fraction is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this product has not been tested in detail, however in double radial immunodiffusion a reaction with Mouse has been obtained.

Attributs du produit: The reactivity of the antiserum is directed to the Fc portion of the subclass IgG1. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG1 in rat serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of rat IgG1 from serum or plasma.

Purification: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Immunogène: Pools of purified homogenous IgG1 isolated from pooled rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Information d'application

Indications d'application: Precipitation assays. In immunoelectrophoresis use 2 μL or equivalent against 120 μL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μL antiserum in a 3 mm diameter centre well and 2 μL serum samples (neat and diluted) in 2 mm diameter peripheral wells.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum. No preservative added as it may interfere with he antibody activity. Total protein and IgG concentration in the antiserum are comparable to those of pooled normal sheep serum. No foreign

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Détail du antigène

Antigène: IgG1

Classe de substances: Antibody