Chèvre anti-Rat IgG2b (Subclass specific) Anticorps - Preadsorbed

N° du produit ABIN458752

Aperçu rapide pour Chèvre anti-Rat IgG2b (Subclass specific) Anticorps - Preadsorbed (ABIN458752)

Antigène: IgG2b

Clonalité: Polyclonal

Application: ELISA, Western Blotting (WB), Dot Blot (DB), Indirect Immunofluorescence Assay (IFA)

Isotype: IgG

Détail du produit

Épitope: Subclass specific

Reactivité: Rat

Hôte: Chèvre

Fonction: Goat anti Rat IgG2b (subclass specific)

Specificité: IgG fraction of polyclonal Goat antiSerum to Rat IgG2b, subclass specific.

Réactivité croisée (Details): This IgG fraction is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this antiSerum has not been tested in detail.

Attributs du produit: The reactivity of the antiserum is directed to the subclass IgG2b as tested against rat sera and a panel of purified homogenous immunoglobulins. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. As unlabelled primary or secondary reagent for indirect detection of IgG2b at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to prepare conjugates of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.

Purification: Purified IgG fraction from antiserum

Immunogène: Pools of purified homogenous IgG2b isolated from pooled rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Information d'application

Indications d'application: Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: The lyophilized IgG (7S) fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Détail du antigène

Antigène: IgG2b

Classe de substances: Antibody