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Chèvre anti-Humain IgA Anticorps

ELISA, WB, IHC Polyclonal IgG unconjugated F(ab')2 fragment
Rockland
N° du produit ABIN5608046
N° du produit (Fournisseur): 709-1106

Aperçu rapide pour Chèvre anti-Humain IgA Anticorps (ABIN5608046)

Antigène

IgA

Fragment

F(ab')2 fragment

Clonalité

  • 279
  • 67
Polyclonal

Application

  • 174
  • 133
  • 128
  • 101
  • 96
  • 87
  • 51
  • 46
  • 37
  • 33
  • 26
  • 23
  • 17
  • 17
  • 16
  • 14
  • 13
  • 7
  • 7
  • 5
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Isotype

IgG
  • Reactivité

    • 173
    • 54
    • 38
    • 15
    • 14
    • 13
    • 13
    • 12
    • 12
    • 11
    • 9
    • 6
    • 6
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain

    Hôte

    • 182
    • 101
    • 53
    • 17
    • 14
    • 6
    • 2
    Chèvre

    N° du produit (Fournisseur)

    709-1106

    Fournisseur

    Rockland

    Fonction

    F(ab')2 Human IgA (alpha chain) Antibody

    Réactivité croisée (Details)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgA and Human Serum.  No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).  Specificity was confirmed by ELISA at less than 1 % cross reactivity against other human heavy or light chain isotypes.

    Attributs du produit

    F(ab')2 Anti-Human IgG IgA IgM (H&L) Texas Red Antibody generated in goat detects human (heavy and light chain) immunoglobulin G, A, and M. Immunoglobulin G binds to antigens and can neutralize or opsonize targets, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunoglobulin molecules linked through their F(c) domain by the J chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

    Purification

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.  

    Stérilité

    Sterile filtered

    Immunogène

    Optional[Immunogen]: Human IgA alpha heavy chain
  • Indications d'application

    Application Note: Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1.3 mg/mL

    Buffer

    Optional[Buffer]: None, Preservative:None

    Agent conservateur

    Without preservative

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store Secondary Antibody at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.

    Date de péremption

    12 months
  • Antigène

    IgA

    Classe de substances

    Antibody

    Sujet

    F(ab')2 Anti-Human IgA Antibody generated in goat detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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