Chèvre anti-Humain lambda Light Chain (Lambda-IgLC) Anticorps (Biotin)

N° du produit ABIN5608058

N° du produit (Fournisseur): 709-1611

Aperçu rapide pour Chèvre anti-Humain lambda Light Chain (Lambda-IgLC) Anticorps (Biotin) (ABIN5608058)

Antigène: Lambda-IgLC (lambda Light Chain (Lambda-IgLC))

Fragment: F(ab')2 fragment

Clonalité: Polyclonal

Conjugué: Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Isotype: IgG

Détail du produit

Reactivité: Humain

Hôte: Chèvre

N° du produit (Fournisseur): 709-1611

Fournisseur: Rockland

Fonction: F(ab')2 Human (lambda chain) Antibody Biotin Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Biotin, Human IgG and Human Serum.  No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).  Specificity was confirmed by ELISA at less than 1 % cross reactivity against other human heavy or light chain isotypes.

Attributs du produit: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Immunogène: Optional[Immunogen]: Human lambda light chain

Information d'application

Indications d'application: Application Note: This product has been assayed against 1.0 μg of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détail du antigène

Antigène: Lambda-IgLC (lambda Light Chain (Lambda-IgLC))

Autre désignation: lambda light chain

Sujet: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.