Lapin anti-Souris kappa Light Chain Anticorps (Alkaline Phosphatase (AP))

N° du produit ABIN5608088

N° du produit (Fournisseur): 610-4510

Aperçu rapide pour Lapin anti-Souris kappa Light Chain Anticorps (Alkaline Phosphatase (AP)) (ABIN5608088)

Antigène: kappa Light Chain

Clonalité: Polyclonal

Conjugué: Alkaline Phosphatase (AP)

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Isotype: IgG

Détail du produit

Reactivité: Souris

Hôte: Lapin

N° du produit (Fournisseur): 610-4510

Fournisseur: Rockland

Fonction: Mouse (kappa chain) Antibody Alkaline Phosphatase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), and anti-Rabbit Serum.  Solid phase adsorption(s) against a panel of Mouse IgG lambda, IgM lambda and IgA lambda proteins was performed.  Specificity was confirmed by ELISA at less than 1 % cross reactivity against other mouse heavy or light chain isotypes.

Attributs du produit: MOUSE IgM (alpha chain) (RABBIT) Antibody generated in rabbit detects specifically mouse IgM whole molecule.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Stérilité: Sterile filtered

Immunogène: Optional[Immunogen]: Mouse kappa light chain

Information d'application

Indications d'application: Application Note: Anti-Mouse kappa chain Alkaline Phosphatase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:1,000 ELISA Dilution: 1:1,000 - 1:2,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Date de péremption: 12 months

Détail du antigène

Antigène: kappa Light Chain

Sujet: Anti-Mouse κ (kappa chain) (RABBIT) Antibody generated in rabbit detects specifically Mouse kappa light chain. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). The light chains can be categorized into kappa type or lambda type and both are used to construct the antigen binding F(ab) region of an antibody along with the variable region of the heavy chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Mouse IgG kappa is conjugated to alkaline phosphatase.