Chèvre anti-Humain lambda Light Chain (Lambda-IgLC) Anticorps (Alkaline Phosphatase (AP))
Aperçu rapide pour Chèvre anti-Humain lambda Light Chain (Lambda-IgLC) Anticorps (Alkaline Phosphatase (AP)) (ABIN5608089)
Antigène
Clonalité
Conjugué
Application
Isotype
-
-
Reactivité
- Humain
-
Hôte
- Chèvre
-
N° du produit (Fournisseur)
- 609-1511
-
Fournisseur
- Rockland
-
Fonction
- Human (lambda chain) Antibody Alkaline Phosphatase Conjugated
-
Réactivité croisée (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Goat Serum. Specificity was confirmed by ELISA minimal cross reactivity against other human heavy or light chain isotypes.
-
Attributs du produit
- The anti-Human κ (kappa chain) Antibody detects the kappa chain subunit. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). In humans the light chains can be categorized into kappa type or lambda type and both are used to construct the antigen binding F(ab) region of an antibody along with the variable region of the heavy chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Human IgG kappa is conjugated to alkaline phosphatase.
-
Purification
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
-
Stérilité
- Sterile filtered
-
Immunogène
- Optional[Immunogen]: Human lambda light chain
-
-
-
-
Indications d'application
- Application Note: Anti-Human lambda chain Alk Phos conjugate is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized
-
Restrictions
- For Research Use only
-
-
-
Format
- Liquid
-
Concentration
- 1.0 mg/mL
-
Buffer
-
Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide -
Agent conservateur
- Sodium azide
-
Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Stock
- 4 °C
-
Stockage commentaire
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
-
Date de péremption
- 12 months
-
-
- Lambda-IgLC (lambda Light Chain (Lambda-IgLC))
-
Autre désignation
- lambda light chain
-
Sujet
- The anti-Human λ (lambda chain) Antibody detects the lambda chain subunit. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). In humans the light chains can be categorized into kappa type or lambda type and both are used to construct the antigen binding F(ab) region of an antibody along with the variable region of the heavy chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Human IgG lambda is conjugated to alkaline phosphatase.
Antigène
-