Chèvre anti-Furet IgA Anticorps (HRP)

N° du produit ABIN5608099

N° du produit (Fournisseur): 618-103-006

Aperçu rapide pour Chèvre anti-Furet IgA Anticorps (HRP) (ABIN5608099)

Antigène: IgA

Clonalité: Polyclonal

Conjugué: HRP

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Isotype: IgG

Détail du produit

Reactivité: Furet

Hôte: Chèvre

N° du produit (Fournisseur): 618-103-006

Fournisseur: Rockland

Fonction: Ferret IgA (alpha chain) Antibody Peroxidase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Ferret IgA and Ferret Serum.   Specificity was confirmed by ELISA at less than 1 % cross reactivity against other Ferret heavy or light chain isotypes.

Attributs du produit: Anti-Ferret IgG IgA IgM Fluorescein Antibody generated in goat detects immunoglobulin G, A, and M from ferret. Immunoglobulin G binds to antigens and can neutralize or opsonize targets, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunoglobulin molecules linked through their F(c) domain by the J chain.

Purification: Anti-Ferret IgA (alpha chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Ferret IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogène: Optional[Immunogen]: Anti-Ferret IgA was produced by repeated immunization with ferret IgA alpha heavy chain in goat.

Information d'application

Indications d'application: Application Note: Anti-Ferret IgA Peroxidase conjugate is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. Ferret IgA (alpha chain) antibody has been assayed against 1.0 μg of Ferret IgA in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for Anti-Ferret IgA (alpha chain) Antibody. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:10,000 - 1:50,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Thimerosal

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Date de péremption: 12 months

Détail du antigène

Antigène: IgA

Classe de substances: Antibody

Sujet: Anti-Ferret IgA Peroxidase Antibody generated in goat detects immunoglobulin A from ferret. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Anti-Ferret IgA (alpha chain) Antibody is ideal for investigators in Cancer, Immunology, and Microbiology research.