Chèvre anti-Humain IgA, IgG, IgM Anticorps (FITC) - Preadsorbed

N° du produit ABIN5608109

N° du produit (Fournisseur): 709-102-130

Aperçu rapide pour Chèvre anti-Humain IgA, IgG, IgM Anticorps (FITC) - Preadsorbed (ABIN5608109)

Antigène: IgA, IgG, IgM

Fragment: F(ab')2 fragment

Clonalité: Polyclonal

Conjugué: FITC

Application: Flow Cytometry (FACS), Fluorescence Microscopy (FM), FLISA

Isotype: IgG

Détail du produit

Reactivité: Humain

Hôte: Chèvre

N° du produit (Fournisseur): 709-102-130

Fournisseur: Rockland

Fonction: F(ab')2 Human IgG IgA IgM (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed

Réactivité croisée (Details): Minimal crossreactivity against Mouse Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum.  No reaction was observed against anti-Goat IgG F(c), anti-Pepsin or Mouse Serum Proteins.  This product is suitable for the detection of all Human immunoglobulin classes, isotypes and chain combinations.

Attributs du produit: F(ab')2 Anti-Human IgG F(ab')2 Rhodamine Antibody generated in goat detects F(ab')2 from human. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

Purification: This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogène: Optional[Immunogen]: Human IgG, IgA, and IgM whole molecules

Information d'application

Indications d'application: Application Note: F(ab')2 Anti-Human IgG IgA IgM (H&L) Fluorescein Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détail du antigène

Antigène: IgA, IgG, IgM

Autre désignation: IgG,IgA,IgM

Classe de substances: Antibody

Sujet: F(ab')2 Anti-Human IgG IgA IgM (H&L) Fluorescein Antibody generated in goat detects human (heavy and light chain) immunoglobulin G, A, and M. Immunoglobulin G binds to antigens and can neutralize or opsonize targets, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunoglobulin molecules linked through their F(c) domain by the J chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.