Chèvre anti-Souris IgA, IgG, IgM Anticorps (FITC) - Preadsorbed
Aperçu rapide pour Chèvre anti-Souris IgA, IgG, IgM Anticorps (FITC) - Preadsorbed (ABIN5608110)
Antigène
Fragment
Clonalité
Conjugué
Application
Isotype
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Reactivité
- Souris
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Hôte
- Chèvre
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N° du produit (Fournisseur)
- 710-102-130
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Fournisseur
- Rockland
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Fonction
- F(ab')2 Mouse IgG IgA IgM (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed
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Réactivité croisée (Details)
- Minimal crossreactivity against Human Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. No reaction was observed against anti-Goat IgG F(c), anti-Pepsin or Human Serum Proteins. This product is suitable for the detection of all Human immunoglobulin classes, isotypes and chain combinations.
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Attributs du produit
- F(ab')2 Anti-Mouse IgG IgA IgM (H&L) Fluorescein Antibody generated in goat detects reactivity to Mouse IgG, Mouse IgA, and Mouse IgM subclasses and chains. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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Purification
- This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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Immunogène
- Optional[Immunogen]: Mouse IgG IgA and IgM whole molecule
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Indications d'application
- Application Note: F(ab')2 Anti-Mouse IgG IgA IgM (H&L) Fluorescein Antibody has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Flow Cytometry Dilution: User Optimized IF Microscopy Dilution: 1:500 - 1:2,500 Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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Concentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide -
Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Date de péremption
- 12 months
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- IgA, IgG, IgM
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Autre désignation
- IgG,IgA,IgM
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Classe de substances
- Antibody
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Sujet
- F(ab')2 Anti-Mouse IgG IgA IgM (H&L) Fluorescein Antibody generated in goat detects reactivity to Mouse IgG, Mouse IgA, and Mouse IgM subclasses and chains. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
Antigène
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