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Lapin anti-Souris kappa Light Chain Anticorps

WB, ELISA, IHC Polyclonal IgG unconjugated
Rockland
N° du produit ABIN5608150
N° du produit (Fournisseur): 610-4110

Aperçu rapide pour Lapin anti-Souris kappa Light Chain Anticorps (ABIN5608150)

Antigène

kappa Light Chain

Clonalité

  • 42
  • 22
Polyclonal

Application

  • 40
  • 33
  • 26
  • 16
  • 14
  • 14
  • 10
  • 9
  • 9
  • 9
  • 8
  • 5
  • 4
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Isotype

IgG
  • Reactivité

    • 44
    • 17
    • 2
    • 1
    • 1
    Souris

    Hôte

    • 22
    • 19
    • 13
    • 4
    • 3
    • 3
    Lapin

    N° du produit (Fournisseur)

    610-4110

    Fournisseur

    Rockland

    Fonction

    Mouse kappa Antibody

    Réactivité croisée (Details)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum.   Specificity was confirmed by ELISA at less than 1 % cross reactivity against other mouse heavy or light chain isotypes.

    Attributs du produit

    Anti-Mouse IgM mu heavy chain antibody generated in rabbit detects specifically Mouse IgM mu heavy chain.

    Purification

    Anti-MOUSE kappa (kappa chain) (RABBIT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

    Stérilité

    Sterile filtered

    Immunogène

    Optional[Immunogen]: Mouse kappa light chain
  • Indications d'application

    Application Note: Anti-MOUSE kappa (kappa chain) (RABBIT) Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:5,000 - 1:50,000 ELISA Dilution: 1:100,000 Other: User Optimized

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1.0 mg/mL

    Buffer

    Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

    Stabilizer: None

    , Preservative:0.01 % (w/v) Sodium Azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

    Date de péremption

    12 months
  • Antigène

    kappa Light Chain

    Sujet

    Anti-Mouse κ (kappa chain) (RABBIT) Antibody generated in rabbit detects specifically Mouse light chain. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). The light chains can be categorized into kappa type or lambda type and both are used to construct the antigen binding F(ab) region of an antibody along with the variable region of the heavy chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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