Chèvre anti-Rat IgA, IgG, IgM Anticorps (Biotin)

N° du produit ABIN5608151

N° du produit (Fournisseur): 612-106-130

Aperçu rapide pour Chèvre anti-Rat IgA, IgG, IgM Anticorps (Biotin) (ABIN5608151)

Antigène: IgA, IgG, IgM

Clonalité: Polyclonal

Conjugué: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Détail du produit

Reactivité: Rat

Hôte: Chèvre

N° du produit (Fournisseur): 612-106-130

Fournisseur: Rockland

Fonction: Rat IgG IgA IgM (H&L) Antibody Biotin Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rat IgA, Rat IgG and Rat IgM.  This reagent is suitable for the detection of all rat immunoglobulin subclasses, isotypes and chain combinations.

Attributs du produit: Anti-Rat IgG (H&L) generated in goat detects rat Immunoglobulin G. Both the Heavy and Light chains of the antibody molecule are present. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rat IgG is conjugated to biotin.

Purification: This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogène:

Immunogen: Anti-Rat IgG IgA IgM (H&L) was produced by repeated immunization with Rat IgG, IgA and IgM whole molecules in goat.

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: Anti-Rat IgG IgA IgM (H&L) Biotin conjugated antibodies has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:10,000 Western Blot Dilution: 1:2,000 - 1:20,000 ELISA Dilution: 1:150,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détail du antigène

Antigène: IgA, IgG, IgM

Autre désignation: IgG IgA IgM

Classe de substances: Antibody

Sujet: Anti-Rat IgG IgA IgM (H&L) biotin conjugated antibody generated in goat detects specifically rat IgG IgA IgM (H&L). This secondary biotin conjugated antibody anti-Rat is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.