Immunoaffinity Chromatography. This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose.
ELISA, IHC, WB
Polyclonal
IgG
HRP
F(ab')2 fragment
Indications d'application
Optimal working dilutions should be determined experimentally by the investigator.
Restrictions
For Research Use only
Reconstitution
Restore with 1.0 mL of deionized water (or equivalent).
Concentration
2.0 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 10 mg/mL BSA (Immunoglobulin and Protease free) as stabilizer and 0.01 % (w/v) Gentamicin Sulfate as preservative.
Conseil sur la manipulation
Avoid repeated freezing and thawing. Dilute only prior to immediate use Centrifuge product if not completely clear after standing at room temperature. Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
Carvalho, Walter, Baermann-Stapel, Weller, Panne, Schenk, Schneider: "Non-invasive monitoring of immunization progress in mice via IgG from feces." dans: In vivo (Athens, Greece), Vol. 26, Issue 1, pp. 63-9, (2012) (PubMed).