Aperçu rapide pour Poulet anti-Chévre IgG (Heavy & Light Chain) Anticorps (Alkaline Phosphatase (AP)) (ABIN619193)
Antigène
IgG
Clonalité
Polyclonal
Conjugué
Alkaline Phosphatase (AP)
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC)
Isotype
IgY
Épitope
Heavy & Light Chain
Reactivité
Chévre
Hôte
Poulet
Réactivité croisée
Humain, Souris, Lapin
Attributs du produit
Chicken anti-goat IgG (H&L), ALP (alkaline phosphatase) conjugated, is a secondary antibody which binds to goat IgGs in immunological assays. Antibodies are adsorbed against human, mouse, rabbit IgG/serum and are affinity purified using solid phase goat IgG.
1:500-1:2000 for ELISA/western blot, 1:20-1: 2000 for immunohistochemistry (IHC), 1:50-1: 5000 for immunocytochemistry (ICC).
Restrictions
For Research Use only
Format
Liquid
Conseil sur la manipulation
Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Do not add Sodium azide. Each reagent is stable for the period shown on the bottle label if stored as directed. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.
Stock
4 °C
Stockage commentaire
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.