Chèvre anti-Humain IgG (Fc Region) Anticorps (Alkaline Phosphatase (AP))

N° du produit ABIN619623

Détail du produit

Antigène: IgG

Fragment: F(ab')2 fragment

Épitope: Fc Region

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC)

Réactivité croisée: Mouton, Souris, Lapin

Homologie: human IgG Fc (two heavy chains with constant domains)

Attributs du produit: Goat anti-human IgG Fc (two heavy chains with constant domains) F(ab) '2 fragment is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.
F(ab)'2 fragment

Purification: Affinity purification

Immunogène: Purified human IgG

Isotype: IgG

Information d'application

Indications d'application: 1: 500 - 2 000 (ELISA) and (WB), 1: 20 -1: 2000 (IHC), 1: 50 - 1: 5000 (ICC)

Commentaires:

This antibody reacts with the heavy (gamma) chains on human IgG based on immunoelectrophoresis.No reactivity is observed with the light chains on human immunoglobulins, or non-immunoglobulin human serum or bovine, mouse or rabbit serum proteins or mouse and rabbit IgG based on immunoelectrophoresis.

Restrictions: For Research Use only

Stockage

Format: Liquid

Conseil sur la manipulation: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Each reagent is stable for the period shown on the bottle label if stored as directed.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.

Stock: 4 °C

Stockage commentaire: Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody