Chèvre anti-Humain IgM (Chain mu) Anticorps (HRP)

N° du produit ABIN619752

Aperçu rapide pour Chèvre anti-Humain IgM (Chain mu) Anticorps (HRP) (ABIN619752)

Antigène: IgM

Fragment: F(ab')2 fragment

Clonalité: Polyclonal

Conjugué: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Détail du produit

Épitope: Chain mu

Reactivité: Humain

Hôte: Chèvre

Réactivité croisée: Humain

Attributs du produit: Goat anti-human IgM, µ chain, F(ab) '2 fragment is a HRP conjugated secondary antibody which is reacting with human IgM, µ chain, F(ab) '2. Antibody solution may contain small amounts of intact IgG.
F(ab)'2 fragment

Purification: Affinity purification

Pureté: > 90 % based on SDS-PAGE.

Information d'application

Indications d'application: 1:500-1:5000 for immunohistochemistry (IHC), 1:10 000-1:50 000 for ELISA/western blot.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution please add 0.55 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.

Conseil sur la manipulation: Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.

Stock: 4 °C

Stockage commentaire: Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Détail du antigène

Antigène: IgM

Classe de substances: Antibody